Composite

Part:BBa_K594010

Designed by: Yu Xiaoyan   Group: iGEM11_OUC-China   (2011-10-01)


a 3-O-C12-HSL induced composite promoter

This composite promoter is composed of ptetR(R0040), RBS(B0034), lasR(C0079), double terminator(B0015),plas(R0079). The ptetR have a constitutivel expression under the condition that no tetR exists, producing lasR. When the lasR unites with 3-O-C12-HSL and form a complex bound to the cassette in plas, the downstream genes' expression can be activated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1064
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 402
    Illegal AgeI site found at 599
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None